GRP-78, Heat Shock 70 kDa Protein 5 (Glucose-Regulated Protein, 78kDa), GRP78, Endoplasmic Reticulum Lumenal Ca(2+)-Binding Protein Grp78, Immunoglobulin Heavy Chain-Binding Protein, BIP, Heat Shock 70kD Protein 5 (Glucose-Regulated Protein, 78kD), Epididymis Secretory Sperm Binding Protein Li 89n, 78 KDa Glucose-Regulated Protein, Heat Shock 70 KDa Protein 5, HEL-S-89n, GRP-78, MIF2
Recombinant human 78 kDa glucose-regulated protein, derived from transfected human HEK293 cells.
Concentration:
Determined by Pierce BCA protein assay kit
Molecular Weight:
Recombinant human HSPA5 protein has a calculated molecular mass of 70 kDa. Due to the abundant glycosylation, it migrates as approximately 80-100 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Tag:
His
Expression System:
HEK293 cells
Purity:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Form:
Lyophilized powder
Sequence:
Glu19-Leu654
Formula:
Lyophilized from a 0.2 µm filtered solution in PBS
Application Notes:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
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