Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Gamma, Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase, Activation Protein, Gamma Polypeptide, Protein Kinase C Inhibitor Protein 1,14-3-3 Gamma.
Recombinant human 14-3-3 protein gamma with an N-terminal His-tag, derived from the transfected human HEK293 cells.
Concentration:
Determined by Pierce BCA protein assay
Molecular Weight:
Recombinant protein product has a calculated molecular mass of 28 kDa. Due to the abundant glycosylation, it migrates as two major bands (28 kDa, 35 kDa) in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image b
Tag:
His
Expression System:
HEK293 cells
Purity:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Form:
Lyophilized powder
Sequence:
Val2-Asn247
Formula:
Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4) with 1% mannitol and 5% trehalose
Application Notes:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
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