Recombinant human interferon, gamma with an N-terminal His-tag, derived from the transfected human HEK293 cells.
Concentration:
Determined by Pierce BCA protein assay
Molecular Weight:
Recombinant protein product has a calculated molecular mass of 17 kDa. Due to the abundant glycosylation, it migrates as approximately 20-25 kDa major protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analy
Tag:
His
Expression System:
HEK293 cells
Purity:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Form:
Lyophilized powder
Sequence:
Gln24-Gln166
Formula:
Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4) with 1% mannitol and 5% trehalose
Application Notes:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
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