Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2 (TNF-a).
Recombinant Rat TNFa with a C-terminal His-tag, derived from the transfected human HEK293 cells.
Concentration:
Refer to the label on the vial for the specific concentration, determined by the Pierce BCA Protein Assay Kit.
Molecular Weight:
Recombinant protein product has a calculated molecular mass of 22 kDa. Due to the abundant glycosylation, it migrates as approximately 26 kDa extra protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Tag:
C-terminal His-tag
Expression System:
HEK293 cells
Purity:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Form:
Liquid
Sequence:
G57-L235
Formula:
Filtered solution in 15 mM Na2HPO4 (pH 7.4), 5 mM NaH2PO4, 0.5 M NaCl, 750 mM imidazole, 20% glycerol.
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