Immunogen was Human beta2 Microglobulin. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G affinity chromatography.
Mouse anti-B2M Biotinylated Antibody, 50 ug
Clonality:
Monoclonal
Isotype:
IgG1
Tag:
Biotin
UniProt:
NP_001117171
Application Notes:
ELISA (recommended work dilution= 1:10e6(at least detecting 10 µg/ml)), Western Blotting (recommended work dilution= 1:1000-2000(at least)), Immunohistology-frozen, Apoptosis Induction (recommended work dilution= study in vitro and vivo), (see image)Immunodetection Analysis: The membrane blot was probed with anti- beta2 M primary antibody(1 µg/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular recombinant beta2 M (12 kDa).
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