Hsc70 (Hsp73) Antibody, IgG1, Clone: [N27F3-4], Mouse, Monoclonal Preis auf Anfrage

Article Name:	Hsc70 (Hsp73) Antibody, IgG1, Clone: [N27F3-4], Mouse, Monoclonal Preis auf Anfrage
Biozol Catalog Number:	ROC-200-301-A28
Supplier Catalog Number:	200-301-A28
Alternative Catalog Number:	ROC-200-301-A28
Manufacturer:	Rockland Immunochemicals
Host:	Mouse
Category:	Antikörper
Application:	FC, IF, IHC, IP, WB
Species Reactivity:	Bovine, C. elegans, Canine, Drosophila, Fish, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Other, Plant, Porcine, Rabbit, Rat, Sheep, Xenopus
Immunogen:	This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with a synthetic peptide corresponding to a region of human Hsc70 (Hsp73) protein.
Conjugation:	Unconjugated
Alternative Names:	Heat shock cognate protein 71-kDa antibody, Heat shock protein 8 antibody, Heat-shock70-KD protein 10, formerly antibody, HSC54 antibody, HSC71 antibody, Hsc54, Hsc71, Hsc73, Hsp71, Hsp73, HspA10, HspA8, LAP1, NIP71, Heat shock cognate 71 kDa protein, Heat shock 70 kDa protein 8

Clonality:	Monoclonal
Concentration:	1.0 mg/mL by UV absorbance at 280 nm
Clone Designation:	[N27F3-4]
Isotype:	IgG1
NCBI:	3312
UniProt:	P11142
Buffer:	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:	Liquid (sterile filtered)
Target:	Human
Antibody Type:	Primary Antibody

Application Dilute:	ELISA: 1:10,000 - 1:50,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,000
Application Notes:	This Protein G purified antibody has been tested for use in western blotting, immunoelectron microscopy, immunohistochemistry and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a doublet band approxima

	Adipocyte TonEBP expression is elevated in obesity and TonEBP-deficient mice resist obesity. aTonEBP mRNA levels in iWAT, eWAT, BAT, muscle, and liver from C57BL/6J mice fed with CD (chow diet, n=5) or HFD (high-fat diet, n=7) for 16 weeks. b Immunoblots (top) and quantification of protein levels (bottom) of TonEBP and Hsc70 in iWAT and eWAT (CD, n=4, HFD, n=6). c Correlation of TONEBP mRNA levels in human subcutaneous adipocytes and BMI (n=15). TonEBP mRNA (d) and representative immunoblots (e, top) and quantification of protein levels (e, bottom) in 3T3-L1 adipocytes transfected with miR-negative control (NC), miR-30b (30b), or miR-30c (30c) (n=4). f-iTonEBP+/delta mice (+/delta) and their TonEBP+/+ littermates (+/+) were fed with CD or HFD as indicated. f Changes in body weight after a switch to HFD (CD+/+, n=7, CD+/delta, n=7, HFD+/+, n=13, HFD+/delta, n=11). g Representative images of animals fed with HFD. h Body weight, height, fat mass, and lean mass (n=8). i Representative images (top) and weight (bottom) of fat pads from HFD-fed animals (n=4). n represents number of biologically independent samples (a-c, i) or animals (f-h) or independent experiments with triplicate (d, e). All data are presented as mean+s.e.m. (a, b, f, h, i) or+s.d. (d, e). AU, arbitrary unit. The p-values are determined by unpaired t-test (a,b, h, i) or one-way ANOVA (d-f). *p<0.05 vs. CD (a), NC (d), or +/+(f, h, i). Source data are provided as a Source Data file Figure provided by CiteAb. Source: Nat Commun, PMID: 31387996.
	TonEBP deficiency promotes energy expenditure and beiging of WAT. HFD-fed animals were analyzed by indirect calorimetry to obtain VO2 (a), VCO2 (b), and heat production (c) (n=4). Rectal temperature (temp.) measured in CD-fed animals at room temperature (n=8) (d) and after exposure to cold up to 6h as indicated (4C) (n=6) (e). f, g mRNA abundance of thermogenic genes (f) and beiging marker genes (g) in iWAT of HFD-fed animals (n=5). h mRNA abundance of thermogenic genes (left, n=10) and immunoblots of UCP-1 and Hsc70 (right, n=3) in iWAT of

	Adipocyte TonEBP expression is elevated in obesity and TonEBP-deficient mice resist obesity. aTonEBP mRNA levels in iWAT, eWAT, BAT, muscle, and liver from C57BL/6J mice fed with CD (chow diet, n=5) or HFD (high-fat diet, n=7) for 16 weeks. b Immunoblots (top) and quantification of protein levels (bottom) of TonEBP and Hsc70 in iWAT and eWAT (CD, n=4, HFD, n=6). c Correlation of TONEBP mRNA levels in human subcutaneous adipocytes and BMI (n=15). TonEBP mRNA (d) and representative immunoblots (e, top) and quantification of protein levels (e, bottom) in 3T3-L1 adipocytes transfected with miR-negative control (NC), miR-30b (30b), or miR-30c (30c) (n=4). f-iTonEBP+/delta mice (+/delta) and their TonEBP+/+ littermates (+/+) were fed with CD or HFD as indicated. f Changes in body weight after a switch to HFD (CD+/+, n=7, CD+/delta, n=7, HFD+/+, n=13, HFD+/delta, n=11). g Representative images of animals fed with HFD. h Body weight, height, fat mass, and lean mass (n=8). i Representative images (top) and weight (bottom) of fat pads from HFD-fed animals (n=4). n represents number of biologically independent samples (a-c, i) or animals (f-h) or independent experiments with triplicate (d, e). All data are presented as mean+s.e.m. (a, b, f, h, i) or+s.d. (d, e). AU, arbitrary unit. The p-values are determined by unpaired t-test (a,b, h, i) or one-way ANOVA (d-f). *p<0.05 vs. CD (a), NC (d), or +/+(f, h, i). Source data are provided as a Source Data file Figure provided by CiteAb. Source: Nat Commun, PMID: 31387996.
	Western blot using Rocklands Protein G purified anti-Hsc70 (Hsp73) antibody shows detection of Hsc70 (Hsp73) in whole cell lysates from heat shocked HeLa cells. The band marked by the double arrowheads corresponds to Hsc70 (Hsp73) at an approximate molecular weight of 72/73 kDa. The primary antibody was used at a 1:1,000 dilution.