0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
Flow Cytometry: 10 µL/10 6 cells (0.1 µg), IF Microscopy: User Optimized
Application Notes:
Anti-CD4 has been tested in Flow Cytometry (Cell Surface) and is useful for Immunofluorescence. Researchers should determine optimal titers for applications that are not stated.
Flow Cytometry of Mouse anti-HUMAN CD3 antibody Fluorescein conjugated. Cells: 10 6 Human peripheral blood lymphocytes. Stimulation: none. Antibody: (Black) FITC Mouse isotype control, (Green) Fluorescein Anti-CD3 mouse secondary antibody using 5 ul.
Cell Surface Flow Cytometry of Mouse anti-HUMAN CD4 antibody Fluorescein conjugated. Cells: 10 6 human PBMC. Propidium iodide negative lymphocyte population gated for analysis. Stimulation: none. Antibody: (GREEN) isotype control antibody, (RED) Fluorescein Anti-CD4 mouse secondary antibody using 10 ul (0.1 ug).
Flow Cytometry of Mouse anti-HUMAN CD4 antibody Fluorescein conjugated. Cells: 10 6 Human peripheral blood lymphocytes. Stimulation: none. Antibody: (GRAY) 1µg FITC Mouse isotype control, (BLUE) Fluorescein Anti-CD3 mouse secondary antibody using 5 ul (1µg).
