Check Out

Product Search

Home
Products
LIF Antibody, Rabbit, Polyclonal

LIF Antibody, Rabbit, Polyclonal

Catalog Number: ROC-600-401-CK5

Images (6)
See all images and image information

Article Name:	LIF Antibody, Rabbit, Polyclonal
Biozol Catalog Number:	ROC-600-401-CK5
Supplier Catalog Number:	600-401-CK5
Alternative Catalog Number:	ROC-600-401-CK5
Manufacturer:	Rockland Immunochemicals
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Anti-LIF antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide near the internal region of human LIF.
Conjugation:	Unconjugated
Alternative Names:	LIF Antibody, CDF, DIA, HILDA, MLPLI, Leukemia inhibitory factor, Differentiation-stimulating factor, LIF

Clonality:	Polyclonal
Concentration:	1 mg/mL by UV absorbance at 280 nm
NCBI:	3976
UniProt:	P15018
Buffer:	0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:	Liquid (sterile filtered)
Target:	Human
Antibody Type:	Primary Antibody

Application Dilute:	ELISA: 1:10,000 - 1:20,000, IF Microscopy: 20 µg/mL, WB: 1-2 µg/mL
Application Notes:	Anti-LIF Antibody has been tested for use in ELISA, Western Blotting and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 22 kDa in Western Blots of specific cell lysates and tissu

	Immunofluorescence Validation of LIF. Cell: Mouse 3T3 Cells. Fixation: 4% paraformaldehyde-fixed. Primary Antibody: LIF at 20 µg/mL. Secondary: goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).
	Western Blot Validation of LIF. Load: 15 µg of mouse 3T3 lysates per lane. Primary Antibody: LIF at (lane A: 1 µg/mL, lane B: 2 µg/mL) for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Western Blot Validation of LIF. Load: 15 µg of human lysates per lane. Lane 1: Caco-2, Lane 2: Daudi, Lane 3: HeLa, Lane 4: HepG2, Lane 5: K562, Lane 6: MCF7, Lane 7: Jurkat, Lane 8: SK-N-SH. Primary Antibody: LIF at 1µg/mL at 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Western Blot Validation of LIF. Load: 15 µg of recombinant protein per lane. Primary Antibody: LIF at (Lane 1: 1 µg/mL, lane 2: 2µg/mL, lane 3: 4 µg/mL) for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Western Blot Validation of LIF.Load: 15 µg of Mouse cell and tissue lysates per lane. Lane 1: 3T3/NIH, Lane 2: Mouse Colon, Lane 3: EL4. Primary Antibody: LIF, (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Western Blot Validation of LIF. Load: 15 µg of rat lung lysates per lane. Primary Antibody: LIF at (lane 1: 1 µg/mL, lane 2: 2 µg/mL) for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

* VAT and and shipping costs not included.
Errors and price changes excepted