Histone H3 [ac Lys4] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 4 of human Histone H3.2.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-Histone H3 Ac Lys4 antibody, H3.3AH3F3H3F3B, H3.3B, H3 histone, family 3A, histone H3.3, MGC87783, MGC87782, H3K4ac
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ChIP: 2-5µg/million cells, IHC: 1:1000, IF Microscopy: 1:1000, WB: 1:500
Application Notes:
Anti-Histone H3 [ac Lys4] antibody is tested for Western Blot, Immunocytochemistry, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4
Immunofluorescence of Rabbit Anti-Histone H3 [ac Lys4] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [ac Lys4] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [ac Lys4] is nuclear and chromosomal. Staining: Histone H3 [ac Lys4] is expressed in green and the nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 566 (red).
Western Blot of Rabbit Anti-Histone H3 [ac Lys4] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [ac Lys4] at 1:500 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15-16 kDa. Other band(s): None.
Chromatin Immunoprecipitation of Rabbit Anti-Histone H3 [ac Lys4] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Histone H3 K4ac and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
Dot Blot of Rabbit Histone H3 [ac Lys4] Antibody. Lane 1: K4 umodified. Lane 2: K4 Me1. Lane 3: K4 Me2. Lane 4: K4 Me3. Lane 5: K4 Ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [ac Lys4] antibody at 1:1000 for 45 min at 4C. Secondary antibody: Dylight(TM)488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C.
Western Blot of Rabbit Anti-Histone H3 [ac Lys4] Antibody. Lane 1: HeLa histone prep. Lane 2: NIH-3T3 prep lysates. Load: 30 µg per lane. Primary antibody: Histone H3 [ac Lys4] at 1:500 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.
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