Histone H3 [Trimethyl Lys4, p Thr6] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic trimethylated/phosphorylated peptides surrounding Lysine 4 and Threonine 6 of human Histone H3.2.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ChIP: 2-5µg/million cells, IHC: 1:50, IF Microscopy: 1:50, WB: 1:500
Application Notes:
Anti-Histone H3 [Trimethyl Lys4, p Thr6] antibody is tested for Western Blot, Immunocytochemistry, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band appr
Dot Blot of Rabbit Histone H3 [Trimethyl Lys4, p Thr6] Antibody. Lane 1: Unmodified. Lane 2: pT6. Lane 3: K4Me1pT6. Lane 4: K4Me2pT6. Lane 5: K4Me3pT6. Load: 3, 1, and 0.3 picomoles of peptide. Primary antibody: Histone H3 [Trimethyl Lys4, p Thr6] antibody at 1:1000 for 45 min at 4C. Secondary antibody: Dylight(TM)488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C.
Western Blot of Rabbit Anti-Histone H3 [Trimethyl Lys4, p Thr6] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Trimethyl Lys4, p Thr6] at 1:500 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.
Immunofluorescence of Rabbit Anti-Histone H3 [Trimethyl Lys4, p Thr6] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Trimethyl Lys4, p Thr6] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [Trimethyl Lys4, p Thr6] is nuclear and chromosomal. Staining: Histone H3 [Trimethyl Lys4, p Thr6] is expressed in green and the nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).
Chromatin Immunoprecipitation of Rabbit Anti Histone H3 [Trimethyl Lys4, p Thr6] Antibody - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 ug of Anti-Histone H3 K4me3pT6 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control, DNA was measured by qRT-PCR and normalized to total input (input=1).
Western Blot of Rabbit Anti-Histone H3 [Trimethyl Lys4, p Thr6] Antibody. Lane 1: HeLa histone preps. Load: 30 µg per lane. Primary antibody: Histone H3 [Trimethyl Lys4, p Thr6] at 1:500 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.
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