DYKDDDDK Tag (Anti-FLAG) Antibody Dylight(TM) 680 Conjugated, DL680, Rabbit, Polyclonal

Article Name:	DYKDDDDK Tag (Anti-FLAG) Antibody Dylight(TM) 680 Conjugated, DL680, Rabbit, Polyclonal
Biozol Catalog Number:	ROC-600-444-383
Supplier Catalog Number:	600-444-383
Alternative Catalog Number:	ROC-600-444-383
Manufacturer:	Rockland Immunochemicals
Host:	Rabbit
Category:	Antikörper
Immunogen:	This affinity purified antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase Cleavage Site (ECS) peptide (D-Y-K-D-D-D-D-K) conjugated to KLH using maleimide. This antibody reacts with FLAGa conjugated proteins.
Conjugation:	DL680
Alternative Names:	rabbit anti DYKDDDDK DyLight(TM) 680 Conjugated, rabbit antibody for the detection of FLAG(TM) conjugated proteins DyLight(TM) 680 Conjugated

Clonality:	Polyclonal
Concentration:	1.0 mg/mL by UV absorbance at 280 nm
Buffer:	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:	Lyophilized
Antibody Type:	Primary Antibody

Application Dilute:	ELISA: 1:10,000 - 1:25,000, FLISA: >1:20,000, IF Microscopy: >1:5,000, WB: 1:10,000 - 1:25,000
Application Notes:	The emission spectra for this DyLight(TM) conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent weste


	Immunofluorescence using the Antibody for the detection of FLAG(TM) conjugated proteins DyLight(TM) 680 Conjugated. Staining of third-instar larvae (L3). (a) Third-instar larvae stained for Dpn (blue), Elav (green), Repo (red), and processed using the optional ProK treatment (Steps 31-35). Inset: High-magnification view of larval CNS. (b) Third-instar larvae stained for the multicolor flip-out antigens that decorate neuronal membranes of a subset of Elav-gal4-57C10-MCFO neurons. Scale bars, 50 µm. Additional details regarding antibodies used can be found in Table 2. Figure 5. PMID: 27906168.
	Immunofluorescence using the Antibody for the detection of FLAG(TM) conjugated proteins DyLight(TM) 680 Conjugated. Staining of second-instar larvae (L2). (a,b) Second-instar larvae stained for the multicolor flip-out antigens that decorate random neuronal membranes of a subset of Elav-gal4-57C10-MCFO-line-6-positive neurons. (c,d) Second-instar larvae stained for the nuclear proteins Dpn (blue), Elav (green), and Repo (red), using the optional Pro-K treatment (Steps 31-35) to increase detection of deep-tissue nuclear antigens. Scale bars, 50 µm. Additional details regarding antibodies used can be found in Table 2. Figure 4. PMID: 27906168.
	Properties of DyLight(TM) Conjugates.
	DyLight(TM) 680 Fluorescence Spectra.
	DyLight(TM) dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight(TM) 680 conjugate. Anti-TNFa was detected using a DyLight(TM) 800 conjugate. The image was captured using the Odyssey Infrared Imaging System developed by LI-COR.