0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Chicken
Antibody Type:
Secondary Antibody
Application Dilute:
FLISA: >1:20,000, IF Microscopy: >1:5,000, WB: >1:10,000
Application Notes:
Anti-Chicken IgG DyLight680 has been tested by dot blot and western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex an
Properties of DyLight(TM) conjugates.
Western Blot of Unconjugated Anti-Chicken IgG/IgY (H&L) (GOAT) Antibody (p/n 603-101-002). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG/IgY (H&L) (GOAT) Antibody (p/n 603-101-002) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab. Chicken F(c) migrates slightly higher.
DyLight(TM) dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight(TM) 549 conjugate. Anti-TNFa was detected using a DyLight(TM) 649 conjugate. The image was captured using the Typhoon(TM) 9410 Imaging System.
Western Blot of DyLight(TM) 680 conjugated Goat anti-Chicken IgG antibody. Lane 1: Chicken IgG (p/n 003-0102). Load: 50 ng per lane. Primary antibody: none. Secondary antibody: DyLight(TM) 680 chicken secondary antibody at 1:5,000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 72 kDa, 28 kDa for Chicken IgY. Other band(s): none.
DyLight(TM) 680 Fluorescence Spectra
* VAT and and shipping costs not included. Errors and price changes excepted
