0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Mouse
Antibody Type:
Secondary Antibody
Application Dilute:
FLISA: >1:20,000, IF Microscopy: >1:5,000, WB: >1:10,000
Application Notes:
Anti-Mouse IgG DyLight 800 Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex anal
DyLight(TM) dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight(TM) 680 conjugate. Anti-TNFa was detected using a DyLight(TM) 800 conjugate. The image was captured using the Odyssey Infrared Imaging System developed by LI-COR.
Dot Blot results of Goat Anti-Mouse IgG Antibody DyLight(TM)800 Conjugate. Dots are Mouse IgG: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Goat Anti-Mouse IgG Antibody DyLight(TM)800 Conjugate at 1ug/mL in MB-070 1hr RT. Imaged with GBox, DyLight(TM) 800 Filter.
Western Blot of Goat anti-Mouse IgG Antibody DyLight 800 Conjugated Pre-absorbed. Lane 1: Mouse IgG. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Goat anti-Mouse IgG Antibody DyLight 800 Conjugated Pre-absorbed at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 25 kDa for Mouse IgG.
DyLight(TM) 800 Fluorescence Spectra
Properties of DyLight(TM) Conjugates.
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