Chicken serum for cell culture, cell culture grade chicken serum, sterile serum from chicken
Concentration:
80 mg/ml by Refractometry
Buffer:
None
Source:
Chicken
Form:
Liquid (sterile filtered)
Application Notes:
pH: normal Immunoelectrophoresis: normal Hemoglobin: normal IgG Concentration: normal
Intranasal infection with HSV-1 induces blood leukocytes infiltration in the CNS of chimeric C57BL/6 mice. Representative micrographs illustrating the localization of GFP+infiltrating cells in the olfactory bulb, the interbrain and the hindbrain of GFP+/-WT mice. Sixteen week-old chimeric mice were infected with HSV-1 by the intranasal route and sacrificed prior to (negative control) and on days 4, 6, 8 and 10 post-infection (5 mice per group). Free-floating sections were washed 3 times for 15 min in potassium PBS (KPBS) and incubated for 30 min in KPBS containing 4% goat or chicken (p/n D102-00-0050) serum, 1% BSA and 0.4% Triton X-100. (A) Brain slices of 25-µm thick were processed for immunohistochemistry staining with a primary polyclonal goat anti-GFP and a secondary Alexa 488-conjugated chicken anti-goat antibodies (green). In non-infected mice (NI), no GFP+cells could be found in the brain parenchyma. Following infection, peripheral leukocytes infiltrated the CNS and could be detectable mainly in the olfactory bulb, the interbrain and the hindbrain. Scale bar 100 µm. Fig 3. PMID: 26700486.
Chicken Serum is used as a supplement to cell culture media. Chicken Serum provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors that promote cell growth and health. Chicken Serum was prepared by removal of fibrinogen through clot formation. Chicken Serum was separated from cellular components and purified through centrifugation and filtration techniques.
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