NFkB Antibody Sampler Kit
Catalog Number:
ROC-K711
- Images (5)
| Article Name: | NFkB Antibody Sampler Kit |
| Biozol Catalog Number: | ROC-K711 |
| Supplier Catalog Number: | K711 |
| Alternative Catalog Number: | ROC-K711 |
| Manufacturer: | Rockland Immunochemicals |
| Category: | Kits/Assays |
| Application: | WB |
| Conjugation: | Unconjugated |
| Alternative Names: | Anti-NFKB p50, Anti-NFKB p65, Rabbit Anti-NFKB cRel, Anti-IKBalpha, Control peptides, antibody sample kits |
| Form: | Liquid (sterile filtered) |
| Application Dilute: | ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:2,000 |
| Application Notes: | Each kit contains one unit of the following products: Anti-NFKB p50 (NFKB1) (RABBIT) Antibody (100 µL) Anti-NFKB p65 (Rel A) (RABBIT) Antibody (100 µL) Anti-NFKB cRel (RABBIT) Antibody (100 µL) Anti-IKBalpha C-terminal (RABBIT) Antibody (100 µL) Anti-RABBIT |
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This product is assembled as a kit. See attached protocol or CofA for further details. |
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Western blot of HeLa cell extract. A predominant band ~65 kDa (arrowhead) corresponding to NFkb p65 Rel A is observed with anti NFkB antibody 100-4165. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:5,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5 each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development. |
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Western blot of HeLa cell extract using p/n 100-4166 Anti-NFKB cRel (RABBIT) Antibody (100 µL) . All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5 each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development. |
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Western blot of HeLa cell extract using Anti-NFKB p50 (NFKB1) Antibody 100-4164. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5 each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development. |
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