NFkB p65 ELISA Kit
Catalog Number:
ROC-KAA065
- Images (6)
| Article Name: | NFkB p65 ELISA Kit |
| Biozol Catalog Number: | ROC-KAA065 |
| Supplier Catalog Number: | KAA065 |
| Alternative Catalog Number: | ROC-KAA065 |
| Manufacturer: | Rockland Immunochemicals |
| Category: | Kits/Assays |
| Application: | ELISA |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | NF-kB Transcription Factor Kit, p65 kit, EIA kit, NF-kB p65 Transcription Factor Assay |
| Application Dilute: | ELISA: 1:100 |
| Application Notes: | Rocklands NF-kB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) repla |
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This product is assembled as a kit. See attached protocol or CofA for further details. |
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NF-kB Transcription Factor Binding Assay Schematic |
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Transcription factor assay absorbance of cell lysates isolated from stimulated (20 ng/mL TNFa for 30 min.) and non-stimulated HeLa cells demonstrating NF-?B (p50) activity. |
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NF-kB Sample Plate Format |
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Abeta-induced changes in the expressions of NF-kappaB p65, and effects of taxifolin and cilostazol in N2a Swe cells.E. Significant decrease in DNA binding activity of NF-kappaB by co-treatment with 10 µM taxifolin and 10 µM cilostazol as compared with taxifolin or cilostazol monotherapy. NF-kappaB p65 DNA binding activities were determined using a colorimetric NF-kappaB p65 transcription factor assay kit (p/n KAA065). Results are the means SEMs of 4 experiments. *P< 0.05, **P< 0.01, ***P< 0.001 vs. Vehicle (Veh),$$$P< 0.001 vs. Cilostazol alone,+++P< 0.001 vs. 10 µM Taxifolin alone,P< 0.001 vs. Vehicle (Veh), P< 0.001 vs. None.Fig 3. PMID: 27977755. |
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