Loading Control Antibody Sampler Kit

Article Name:	Loading Control Antibody Sampler Kit
Biozol Catalog Number:	ROC-KAS-001
Supplier Catalog Number:	KAS-001
Alternative Catalog Number:	ROC-KAS-001
Manufacturer:	Rockland Immunochemicals
Category:	Antikörper
Application:	WB
Immunogen:	VDAC, GAPDH, alpha-Tubulin, HDAC-1, beta-Actin
Conjugation:	Unconjugated
Alternative Names:	Loading Control Antibody Sampler Kit, Rabbit Anti-VDAC/Porin Antibody, Rabbit Anti-GAPDH Antibody, Rabbit Anti-alpha-Tubulin Antibody, Rabbit Anti-HDAC-1 Antibody, Rabbit Anti-beta-Actin Antibody

Buffer:	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:	Liquid (sterile filtered)

Application Dilute:	ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:5,000
Application Notes:	Each kit contains one unit of the following products: Anti-VDAC/Porin (RABBIT) Antibody (25 µL) Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (RABBIT) Antibody (25 µL) Anti-alpha-Tubulin (RABBIT) Antibody (25 µL) Anti-HDAC-1 (RABBIT) Antibody (25

	This product is assembled as a kit. See attached protocol or CofA for further details.
	Western Blot of Rabbit Anti-HDAC-1 Antibody. Lane 1: LNCaP prostate cancer cells. Lane 2: none. Load: 50 µg per lane. Primary antibody: HDAC-1 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55kDa for HDAC-1. Other band(s): none.
	Western Blot of Rabbit Anti-HDAC-1 Antibody. Lane 1: 293 whole cell lysate. Lane 2: none. Load: 35 µg per lane. Primary antibody: HDAC-1 antibody at 1:3,500 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~65 kDa corresponding to human HDAC1. Other band(s): none.
	Western Blot of Rabbit Anti-Alpha Tubulin Antibody. Lane 1: whole cell lysates from mouse brain. Lane 2: rat brain. Lane 3: A431 cells. Lane 4: Jurkat cells. Lane 5: HeLa cells. Load: 35 µg per lane. Primary antibody: Alpha Tubulin antibody at 1:1,200 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~50 kDa corresponding to alpha tubulin (arrowhead). Other band(s): none.
	Western Blot of Rabbit Anti-VDAC/Porin Antibody. Lane 1: rat heart whole cell lysate. Lane 2: none. Load: 35 µg per lane. Primary antibody: VDAC/Porin antibody at 1:1,200 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~32 kDa corresponding to VDAC/Porin (arrowhead). Other band(s): none.
	Western Blot of Rabbit Anti-Beta Actin Antibody. Lane 1: molecular weight. Lane 2: human embryonic kidney 293. Lane 3: human lung carcinoma A549. Lane 4: mouse brain. Load: 35 µg per lane. Primary antibody: Beta Actin antibody at 1:1,500 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~42 kDa corresponding to beta Actin (arrowhead). Other band(s): none.