Human Washed Pooled Cells, Human WPCs, Human Red Blood Cells, Human RBCs, erythrocytes
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Source:
Human
Form:
Liquid
Application Notes:
Human whole blood cells are used for complement titration, adsorption procedures, HA assays and for the preparation of stroma as particulate reagents.
The LapDG system regulates hemagglutination. Hemagglutination assays were performed withV. choleraeO1 El Tor A1552 (A) or O1 classical O395 (B) strains. Either wild-type (WT) or indicated deletion mutants were used. The reciprocal of the lowest fold dilution (hemagglutinin [HA] titer) at which cells were able to agglutinate red blood cells was recorded. The graphs represent the means and standard deviations of HA titers from at least three biological replicates. One-way ANOVA and Dunnetts multiple-comparison test were used to compare the mean of each mutant to WT. Mean differences with aPvalue of 0.05 were deemed significant. ***,P0.001, ****,P0.0001. FIG7. PMID: 31796544.
Mammalian cell toxicity assays. (A) HMPC hemolysis assay. Red blood cells (p/n R407-0050) were diluted to 2% with PBS and then incubated with HMPC over the concentration range 0.125-64µg/ml. (B) Cytotoxicity of HMPC towards HKC-8 cells. HKC-8 cells were treated with increasing concentrations of HMPC for 24hours before assessing viability using a WST-1 assay. (C) Cytotoxicity of HMPC towards HepG2 cells. HepG2 cells were treated with increasing concentrations of HMPC for 24hours before assessing viability using a WST-1 assay. All experiments were repeated twice in triplicate and data show the mean +/- standard deviation. Figure 6. PMID: 29728636.
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