1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Source:
Human
Form:
Liquid
Application Dilute:
WB: User Optimized
Application Notes:
A431 Whole Cell Lysate - EGF Stimulated has been tested by SDS-PAGE and western blot. Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing bu
Coomassie stained SDS-PAGE of 20 µl of Human Derived A431 Whole Cell Lysate (Ready-to-Use) separated in a 4-20% gradient gel under reducing conditions (lane 1). Molecular weight standards are shown in lane 2.
SDS-PAGE of A431 Whole Cell Lysate Stimulated with EGF (50ng/mL). Lane 1: reduced A431 Lysate EGF Stimulated. Lane 2: Opal PreStained Molecular Ladder (p/n MB-210-0500). Lane 3: non-reduced A431 Lysate EGF Stimulated. Load: 10µg. 4-20% Gel, Coomassie Blue Stained.
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