1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Source:
Human
Form:
Liquid (sterile filtered)
Application Dilute:
WB: User Optimized
Application Notes:
W09-000-365 has been tested by SDS-PAGE and western blot. Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating ag
Coomassie stained SDS-PAGE of 20 µg of (1) Hela WCL, (2) Jurkat WCL, (3) HEK 293 WCL, (4) MCF-7 WCL, (5) A 549 WCL, (6) Raji WCL separated using a 4-20% gradient gel under reducing conditions. Molecular weight standards are shown.
SDS-Page of HEK239 Whole Cell Lysate. Lane 1: Reduced HEK239 Whole Cell Lysate [10µg]. Lane 2: Opal Prestained Molecular Weight Marker (MB-210-0500). Lane 3: Non-reduced HEK239 Whole Cell Lysate [10µg]. 4-20% SDS-PAGE gel, Coomassie Stained.
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