1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Source:
Mouse
Form:
Liquid (sterile filtered)
Application Dilute:
WB: User Optimized
Application Notes:
W10-001-377 has been tested by SDS-PAGE and western blot. Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating ag
Western Blot of NIH/3T3 Whole Cell Lysate PDGF Stimulated. Lane 1: NIH/3T3 Whole Cell Lysate PDGF Stimulated. Load: 2 µg per lane. Primary antibody: AKT1 pS473 Antibody at 1:1,000 overnight at 4C. Secondary antibody: HRP mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for AKT1 pS473.
Coomassie stained SDS-PAGE of 30 µg of Mouse Derived NIH/3T3 Whole Cell Lysate (Ready-to-Use) resolved on a 4-20% gradient gel under non-reducing conditions (lane 2). Molecular weight standards are shown in lane 1.
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