WM115 is a tumorigenic (VGP) primary melanoma cell line with competence for metastasis. This cell line was established from a metastatic site (right anterior leg) in a 55-year-old female with superficial spreading melanoma. The subject displayed VGP with a Clark level III tumor with thickness of 2.24mm.This cell line features the specific V600D (Val600Asp) mutation at codon 600 in the BRAF gene. This cell line also expresses PTEN loss of function including hemizygous deletion. This cell line was derived from the same patient as the cell lines WM239A, WM165-1 and WM266-4. WM115 cell line originated from the primary tumor, and WM239A, WM165-1 and WM266-4 were from individual lymph-node metastases. WM115 cell line is wild type for N-RAS, c-KIT, and CDK4 genes. WM115 cells produce xenograft tumors when injected into immunocompromised mice.
Concentration:
1.0 million cells/mL (Count By Hemocytometer)
Buffer:
None
Source:
Human
Form:
Frozen Cell Suspension
Application Notes:
The key applications of these cell lines include genetic studies, xenograft production, drug testing, and drug target discovery. These cell line models can be used in various biological assays, and for identifying critical target genes, and cell signaling pathways.
Human melanoma tumor cells with known gene mutations, disease stage, STR, and RPPA profiling
PPARgamma expression and rosiglitazone impact in human melanoma.D,Expression of PPARgamma in human melanocytes and in melanoma cell lines representative of the disease progression, determined by RT-qPCR (top) and by Western blot (bottom). RT-qPCR: open circles are means of technical triplicates from one experiment. Columns are means of at least two independent experiments. Western blot: two different exposure times (short and long) of the same Western blot are shown, PPARgamma protein was quantified based on the short exposure time (GAPDH, loading control). Fig 1. PMID: 30185551.
Established WM115 viable cell growth in culture using appropriate Tumor Specialized Media with 2%FBS.
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