WM3211 is a tumorigenic (VGP) primary melanoma cell line with competence for metastasis. These cells display fibroblastic morphology in culture. This cell line was established from a metastatic site (lymph node) in a 34-year-old female. This cell line contains a L576P mutation at position 576 in the c-KIT gene. The L576P mutation results in an amino acid substitution at position 576 in KIT, from a Leucine (L) to a proline (P). This mutation occurs within the juxtamembrane domain. Mutant KIT proteins have increased kinase activity and transforming activity in vitro. This cell line is wild type for BRAF, PTEN, N-RAS, and CDK4. WM3211 cells produce xenograft tumors when injected into immunocompromised mice.
Concentration:
1.0 million cells/mL (Count By Hemocytometer)
Buffer:
None
Source:
Human
Form:
Frozen Cell Suspension
Application Notes:
The key applications of these cell lines include genetic studies, xenograft production, drug testing, and drug target discovery. These cell line models can be used in various biological assays, and for identifying critical target genes, and cell signaling pathways.
Human melanoma tumor cells with known gene mutations, disease stage, STR, and RPPA profiling
Established WM3211 viable cell growth in culture using appropriate Tumor Specialized Media with 2%FBS.
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