Cholecystokinin (CCK) BioAssay(TM) ELISA Kit (Mouse)
Catalog Number:
USB-024106
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| Article Name: | Cholecystokinin (CCK) BioAssay(TM) ELISA Kit (Mouse) |
| Biozol Catalog Number: | USB-024106 |
| Supplier Catalog Number: | 024106 |
| Alternative Catalog Number: | USB-024106-96 |
| Manufacturer: | US Biological |
| Category: | Kits/Assays |
| Cholecystokinin (CCK) BioAssay(TM) ELISA Kit (Mouse) is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of CCK in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 12.35 -1000pg/ml Sensitivity: 4.82pg/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *024106A: Microtiter Plate, 96 wells, Pre-coated, ready to use *024106B: Standard, 2x1vial 024106C: Standard Diluent, 1x20ml *024106D: Detection Reagent A (green) , 1x1vial *024106E: Detection Reagent B (red), 1x120ul 024106F: Assay Diluent A, 1x12ml 024106G: Assay Diluent B, 1x12ml 024106H: TMB Substrate, 1x9ml 024106K: Stop Solution, 1x6ml 024106L: Wash Buffer, 30X, 1x20ml 024106M: Detection Reagent A Diluent, 1x300ul Precaution: The Stop Solution (024106K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *024106A, *024106B, *024106D and *024106E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37C. 3. Aspirate and wash 3 times. 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 5. Aspirate and wash 5 times. 6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37C. 7. Add 50ul Stop Solution. Read absorbance at 450nm immediately |
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