The PDE4D BioAssay(TM) ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PDE4D protein expression profile in cells. It can be used for measuring the relative amounts of PDE4D in cultured cells as well as screening for the effects that various treatments, inhibitors (i.e. siRNA, or chemicals) or activators have on PDE4D. Sensitivity: >5000cells/well Kit Components: 472755A: Microtiter Strips, 1x96 wells 472755B: TBS, 10X, 1x24ml 472755C: Quenching Buffer, 1x24ml 472755D: Blocking Buffer, 1x50ml 472755E: Wash Buffer, 15X, 1x50ml *472755F: PDE4D Pab, 100X, 1x60ul *472755G: GAPDH Mab, 100X, 1x60ul 472755H: IgG Pab (HRP), 1x6ml 472755J: IgG Mab (HRP), 1x6ml 472755K: Primary Antibody Diluent, 1x12ml 472755L: Substrate, 1x12ml 472755M: Stop Solution (2N Sulfuric Acid), 1x12ml 472755N: Crystal Violet Solution, 1x6ml *472755P: SDS Solution, 1x24ml Storage and Stability: Store all components at 4C. Reconstituted *472755F and *472755G are stable for 2 weeks at 4C.. *472755P should be stored at RT or warmed up to RT if stored at 4C. Other components are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
