Sample Type: Cell line of choice Intended Use: The Phospho-Akt1 (Ser473) Colorimetric Cell-Based ELISA Kit allows for qualitative detection of Phospho-Akt1(Ser473) in plated and fixed cells. Sensitivity: >5000cells/well Assay Time: 4.5 hours Reactivity: Human:S473, Mouse:S473, Rat:S473 Detection Method: Colorimetric 450 nm Format: Cell-Based ELISA Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.