Human CD154 (CD40 Ligand) is a member of the tumor necrosis factor (TNF) family and is expressed on the surface of activated T cells. Interaction of CD154 and CD40 is essential for isotype switching in B cells. Known genetic defects that alter this interaction lead to impaired immune system function. CD154 has been shown to be hyperexpressed by B and T cells in SLE patients. Functional Application: The antibody will block MLR, sgp39 induced human B cell proliferation and T cell dependent B cell differentiation. Applications: Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested. Recommended Dilution Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clonality:
Monoclonal
Clone Designation:
[24-31]
Isotype:
IgG1
Purity:
Purified by Protein A affinity chromatography.
Form:
Supplied as a liquid in FBS, 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate.
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