CD154 is expressed predominantly on activated CD4+ T lymphocytes, and acts as a costimulatory molecule in B cell differentiation/proliferation, immunoglobulin switching and germinal centre formation, through interaction with the CD40 receptor on B cells, and cytokines secreted by activated T cells. Applications: Suitable for use in FLISA, Immunohistochemistry (paraffin), and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 2.5-5.0ug/ml FLISA (indirect), 0.5-2.0ug/ml. Western Blot: 0.1-0.2ug/ml Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Applications are based on unconjugated antibody.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
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