Purified splenic NK cells from the LEW rat strain.
Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein, NKR-P1A or D161, is classified as a type II membrane protein because it has an external C terminus. NKR-P1A, the receptor encoded by the KLRB1 gene, recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand. Human CD161 is a homodimeric disulfide-linked type II membrane bound glycoprotein found mainly on NK cells. CD161 has been implicated in the regulation of NK cell mediated cytotoxicity. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:10, 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: X63-Ag8-653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months after receipt at 4C. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.
Purified by Protein G affinity chromatography from tissue culture supernatant.
Form:
Supplied as a lyophilized powder in PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with R-Phycoerythrin (PE).
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