CD163, a 120kD cell surface glycoprotein is expressed on cells of the monocyte/macrophage lineage. The expression levels of CD163 vary during the course of macrophage differentiation. The highest levels of CD163 expression are found on tissue macrophages but bone marrow derived cells are CD163 negative. Reported to inhibit both African swine fever infection and viral particle binding to alveolar macrophages in a dose-dependent manner. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Westen Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Western Blot: Non-reducing conditions Optimal dilutions to be determined by the researcher. Hybridoma: X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
