CD163 (CD163 Antigen, Hemoglobin Scavenger Receptor, M130, M130 Antigen Precursor, Macrophage-associated Antigen, MM130, Scavenger Receptor Cysteine-rich Type 1 Protein M130) (MaxLight 550), IgG1, Clone: [10B837 2A10/11], Mouse, M
Biozol Catalog Number:
USB-C2548-90CA-ML550
Supplier Catalog Number:
C2548-90CA-ML550
Alternative Catalog Number:
USB-C2548-90CA-ML550-100
Manufacturer:
US Biological
Host:
Mouse
Category:
Antikörper
Application:
FC, IF, IHC, IP, WB
Immunogen:
Porcine alveolar macrophages.
MaxLight(TM)550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor(TM)546, 555, DyLight(TM)549 , Cy3(TM), TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm), Emission (575nm), Extinction Coefficient 150,000. CD163, a 120kD cell surface glycoprotein is expressed on cells of the monocyte/macrophage lineage. The expression levels of CD163 vary during the course of macrophage differentiation. The highest levels of CD163 expression are found on tissue macrophages but bone marrow derived cells are CD163 negative. Reported to inhibit both African swine fever infection and viral particle binding to alveolar macrophages in a dose-dependent manner. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Westen Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Western Blot: Non-reducing conditions Optimal dilutions to be determined by the researcher. Hybridoma: X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
