CD163 is a 120kD cell surface glycoprotein that is expressed on cells of the monocyte/macrophage lineage. The expression levels of CD163 vary during the course of macrophage differentiation. The highest levels of CD163 expression are found on tissue macrophages but bone marrow derived cells are CD163 negative. Reported to inhibit both African swine fever infection and viral particle binding to alveolar macrophages in a dose-dependent manner. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat-1:10, 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: X63-Ag.8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months after receipt at 4C. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.
Purified by Protein A affinity chromatography from tissue culture supernatant.
Form:
Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 5% sucrose, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.
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