The CD163 cell surface glycoprotein is a 175kD molecule also known as ED2. CD163 is expressed by ~50% of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells. May be used in histology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde). Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Western Blot, Immunofluorescence and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells in 100ul Immunohistochemistry (Frozen): 1:50-1:100 Immunohistochemistry (Paraffin): Requires antigen retrieval by protein digestion (trypsin or pronase). Reported reaction following PLP fixation (Periodate-lysine-paraformaldehyde) Optimal dilutions to be determined by the researcher. Positive Control for Immunohistochemistry: Liver Hybridoma: SP2/0-Ag 14 myeloma cells with spleen cells from BALB/c mice Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[10B838 ED2]
Isotype:
IgG1
Purity:
Purified by Protein A affinity chromatography from tissue culture supernatant.
Form:
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
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