MaxLight(TM)490 is a new Blue-Green photostable dye conjugate comparable to DyLight(TM)488, Alexa Fluor(TM)488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm), Emission (515nm), Extinction Coefficient 73,000. The CD163 cell surface glycoprotein is a 175kD molecule also known as ED2. CD163 is expressed by ~50% of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells. May be used in histology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde). Applications: Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry, Immunofluorescence and Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Paraffin): Requires antigen retrieval by protein digestion (trypsin or pronase). Reported reaction following PLP fixation (Periodate-lysine-paraformaldehyde) Immunohistochemistry: frozen sections Optimal dilutions to be determined by the researcher. Positive Control for Immunohistochemistry: Liver Hybridoma: SP2/0-Ag 14 myeloma cells with spleen cells from BALB/c mice Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[10B838 ED2]
Isotype:
IgG1
Purity:
Purified by Protein A affinity chromatography from tissue culture supernatant
Form:
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight(TM)490.
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