CD177 antigen (neutrophil glycoprotein NB1) is expressed by 97% of the caucasian population. Antibodies against NB1 can be involved in neonatal alloimmune neutropenia (NAN). Applications: Suitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: Non-reducing conditions Optimal dilutions to be determined by researcher. Recommended Negative Controls: I1904-78R: IgG1 Murine Negative Control Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
