MaxLight(TM)650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor(TM)647, DyLight(TM)649, Cy5(TM) and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm), Emission (676nm), Extinction Coefficient 250,000. CD177 antigen (neutrophil glycoprotein NB1) is expressed by 97% of the caucasian population. Antibodies against NB1 can be involved in neonatal alloimmune neutropenia (NAN). Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: Non-reducing conditions Optimal dilutions to be determined by researcher. Recommended Negative Controls: I1904-78R: IgG1 Murine Negative Control Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
