CD200 is expressed by a subset of B lymphocytes, some endothelial cells and by neurons. Recent studies have suggested that the CD200-CD200 ligand system is of importance in the control of macrophage and granulocyte activation. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Recommended Secondary Reagents (FC): I1904-09G IgG, H&L X-adsorbed (FITC) Recommended Secondary Reagents (IHC): I1904-65W: IgG, F(ab)2 (HRP) Pab RbxMo I1904-66C: IgG, F(ab)2, X-Adsorbed (HRP) Pab RbxMo Recommended Control: I1904-78R: IgG1 Murine Negative Control Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
