CD203a, originally clustered as SWC9 at the Second International Swine CD Workshop and later identified as the porcine homologue of human ecto-nucleotidepyrophosphatase /phosphodiesterase 1 or NPP1. CD203a is expressed widely in macrophage populations with notably high levels on alveolar macrophages, it is not expressed on monocyte populations. SWC1a, expressed at very much higher levels on monocytes than mature macrophages and CD203a (SWC9), expressed exclusively on mature tissue macrophages have been used as markers of monocyte-macrophage differentiation. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:25-1:200, 10ul labels 10e6 cells in 100ul Immunoprecipitation: Migrates as a homodimer of ~260kD under non-reducing conditions and a 130kD monomer under reducing conditions from preparations of porcine alveolar macrophages. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[10B1007 PM18-7]
Isotype:
IgG1
Purity:
Purified by Protein G affinity chromatography from tissue culture supernatant.
Form:
Supplied as a liquid in PBS, 0.09% sodium azide.
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