CD203a, originally clustered as SWC9 at the Second International Swine CD Workshop and later identified as the porcine homologue of human ecto-nucleotidepyrophosphatase /phosphodiesterase 1 or NPP1. CD203a is expressed widely in macrophage populations with notably high levels on alveolar macrophages, it is not expressed on monocyte populations. SWC1a, expressed at very much higher levels on monocytes than mature macrophages and CD203a (SWC9), expressed exclusively on mature tissue macrophages have been used as markers of monocyte-macrophage differentiation. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunoprecipitation: Migrates as a homodimer of ~260kD under non-reducing conditions and a 130kD monomer under reducing conditions from preparations of porcine alveolar macrophages. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[10B1007 PM18-7]
Isotype:
IgG1
Purity:
Purified by Protein G affinity chromatography from tissue culture supernatant.
Form:
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
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