Human CD221 is a 155kD receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor). CD221 is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signalling proteins. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 1:10. Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry (Paraffin): 1:10-1:40. Protein digestion is required Optimal dilutions to be determined by the researcher. Immunohistochemistry Positive Control: Pancreas, placenta Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control Hybridoma: BALB/c mice were fused with cells of the SP2/0 myeloma cell line Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
