Applications: Suitable for use in FLISA (Fluorescent) and Immunofluorescence. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Label (FITC): Molecular Weight: 390D Absorption Wavelength: 495nm Emission Wavelength: 528nm FITC/Protein Ratio: ~3 moles FITC per mole of IgG Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by salt fractionation and ion exchange. Analysis by IEP resulted in a single precipitin arc against anti-fluorescein, anti-hamster IgG, and anti-hamster serum.
Form:
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA, 0.09% sodium azide. Labeled with FITC.
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