Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG Fab molecule Applications: Suitable for use in Western Blot, ELISA, Immunohistochemistry and immunomicroscopy. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by delipidation, salt fractionation and ion exchange chromatography, followed by pepsin digestion and dialysis.
Form:
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), and 0.01% sodium azide. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
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