Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495nm Emission Wavelength: 528nm Fluorochrome/Protein Ratio: 4.0 moles FITC per mole of Swine IgG Applications: Suitable for use in FLISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications have not been tested. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by ion exchange chromatography. IEP Assay shows a single precipitin arc against source serum protein and IgG.
Form:
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.09% sodium azide. Labeled with FITC. Reconstitution: Reconstitute with 1ml 40-50% glycerol, ddH2O.
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