Recombinant protein corresponding to Ala20-Ser511 from mouse Macrophage Colony Stimulating Factor Receptor, expressed in NS0 cells. Uniprot/Swiss Accession: P09581
M-CSF R (macrophage colony stimulating factor receptor, c-fms) is a 165kD, 977aa type I membrane glycoprotein. It is a member of the type III subfamily of receptor tyrosine kinases that contain five extracellular immunoglobulin-like domains and a cytosolic split kinase domain. M-CSF receptor is expressed primarily on monocytes, macrophages, dendritic cells, and stem cells, and regulates lineage decisions and maturation of these cells. The mouse M-CSF R extracellular domain shares >99% aa identity with rat and 60-63% aa identity with human, dog, cat and cow M-CSF R. It can be released by TACE/ADAM17 cleavage. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 10e6 cells using mouse reticulum cell sarcoma macrophage cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
