Recombinant protein corresponding to human Macrophage Derived Chemokine expressed in E. coli
Human Macrophage-Derived Chemokine (MDC) is a unique member of the CC chemokine family. Human MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells or several lines of epithelial, endothelial or fibroblast origin. Recombinant Human MDC is an 8kD protein containing 67aa residues. Applications: Suitable for use in ELISA. Other applications have not been tested. Recommended Dilutions: ELISA (Sandwich): 2-4ug/ml (100ul/well) detects at least 100pg/ml of recombinant human Macrophage Derived Chemokine when used with M1201-54, Macrophage Derived Chemokine (MDC) (Biotin) as detection antibody at a concentration of 1-2ug/ml. Optimal dilutions to be determined by the researcher. Hybridoma: ICR F1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clonality:
Monoclonal
Clone Designation:
[4i23 1.3_1C6-1D3]
Purity:
Purified by Protein G affinity chromatography from cell culture
Form:
Supplied as a lyophilized powder from PBS. No preservatives added. Reconstitute with 500ul sterile ddH2O.
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