MDC (macrophage-derived chemokine) is a 69-amino acid CC chemokine with an NH2-terminal sequence of 24 residues as a signal sequence. It was originally cloned from human monocyte-derived macrophages1. Recombinant MDC attracts monocyte-derived dendritic cells, activated lymphocytes and natural killer cells. MDC has anti-HIV-1 activity. Expression of MDC mRNA in macrophages is enhanced by LPS, IL-1b and TNFa. MDC is a functional ligand for CCR4. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by Protein A affinity chromatography.
Form:
Supplied as a lyophilized powder, 0.09% sodium azide. Reconsitute with 200ul sterile PBS.
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