Recombinant protein corresponding to rat MIP-1 alpha, expressed in E.coli.
MIP-1 alpha is also known as CCL3. MIP-1 alpha has a molecular weight of approximately 7.8kD and is produced by activated macrophages. MIP-1 alpha has been shown to be involved in the inflammatory response of blood monocytes and tissue macrophages. Applications: Suitable for use in ELISA, Western Blot and Functional Assays. Other applications not tested. Recommended Dilutions: ELISA: 0.5ug/ml Western Blot: 0.1-0.2ug/ml Optimal working dilutions to be determined by the researcher. Recommended Secondary Reagents: Sheep anti Rabbit IgG: HRP Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by affinity chromatography from whole serum.
Form:
Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with 100ul sterile ddH2O.
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