Recombinant protein corresponding to rat MIP-1 alpha, expressed in E.coli.
MaxLight(TM)490 is a new Blue-Green photostable dye conjugate comparable to DyLight(TM)488, Alexa Fluor(TM)488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm), Emission (515nm), Extinction Coefficient 73,000. MIP-1 alpha is also known as CCL3. MIP-1 alpha has a molecular weight of approximately 7.8kD and is produced by activated macrophages. MIP-1 alpha has been shown to be involved in the inflammatory response of blood monocytes and tissue macrophages. Applications: Suitable for use in FLISA, Western Blot and Functional Assays. Other applications not tested. Recommended Dilutions: Optimal working dilutions to be determined by the researcher. Recommended Secondary Reagents: Sheep anti Rabbit IgG: HRP Storage and Stability: May be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Applications are based on unconjugated antibody.
Purity:
Purified by affinity chromatography from whole serum.
Form:
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight(TM)490.
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