Recombinant protein corresponding to rat Macrophage Inflammatory Protein-1 alpha, expressed in E. coli
Both MIP-1 alpha and MIP-1 beta are structurally and functionally related CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes their effect on lymphocytes differ, with MIP-1 alpha selectively attracting CD8+ lymphocytes and MIP-1 beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1 alpha and MIP-1 beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1 alpha and MIP-1 beta are active on human and murine hematopoietic cells. Applications: Suitable for use in ELISA, Western Blot and Neutralization. Other applications not tested. Recommended Dilution: ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution. Detects 0.2-0.4ng/well of recombinant rat MIP-1a. Western Blot: 0.1-0.2ug/ml. The dectection limit is 1.5-3ng/lane, under reducing or non-reducing conditions. Neutralization: 8-10ug/ml yields one-half maximal inhibition (ND50) of the biological activity of rat MIP-1a (100ng/ml). Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by Immunoaffinity chromatography.
Form:
Supplied as a lyphilized powder from PBS. Reconstitute with sterile ddH2O.
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