Recombinant protein corresponding to Gly23-Gly104 from mouse CCL12 expressed in E. coii.
CCL12, also called MCP-5, is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. Mouse CCL12 encodes a 104aa residue precursor protein with a 22aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82aa residue mature protein. Recombinant CCL12 is a potent chemoattractant for monocytes and lymphocytes but not neutrophils. CCL12 has been found to be a functional ligand for CCR2. Applications: Suitable for use in ELISA and Neutralization. Other applications not tested. Recommended Dilution: Neutralization: Measured by its ability to neutralize CCL12/MCP-5-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.5-2.5ug/ml in the presence of 0.2ug/ml Recombinant Mouse CCL12/MCP-5. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
